Pdf evaluation of the bactec mgit 960 tb 0 system for. This was a prospective descriptive study comparing the performance of the lpa hain lifescience, nehren, germany to bactec mgit 960 system becton dickinson microbiology system, sparks, md, usa for susceptibility testing of first and secondline antitb drugs. Evaluation of the effectiveness of bactec mgit 960 for the. The four drugs studied were levofloxacin, amikacin, capreomycin, and ethionamide. Bactec mycobacterium growth indicator tube mgit 960 system. Evaluation of the bactec mgit 960 in comparison with bactec 460 tb for detection and recovery of mycobacteria from clinical specimens. For sm, 78 and 72% of the results obtained by the mgit 960 and the manual mgit systems, respectively, agreed with the bactec 460 results. Wear uv protective glasses when observing fluorescence and use only longwave illumination 365 nm. Its performance was compared to those of the radiometric bactec 460 instrument and eggbased lowensteinjensen.
The bactec mgit 960 system for testing susceptibility to secondline drugs was evaluated with 117 clinical strains in a multicenter study. The instrument scans the mgit every 60 minutes for increased fluorescence. Note, that the bactec mgit 960 instrument does not request data from an lis. Comparison between the bactec mgit 960 system and the agar. The objective of the study was to compare the manual mycobacteria growth indicator tube mgit method and the bactec mgit 960 system to the bactec 460 method for susceptibility testing of mycobacterium tuberculosis. Bd bactec mgit automated mycobacterial detection system. This infectious disease still kills an estimated 3 million persons a year worldwide, making it the.
Pdf mgit procedure manual for bactec mgit 960 tb system. Appropriate solutions of drugs were added in a volume of 0. Evaluation of the bactec mgit 960 tb system for recovery and. Original article the manual mgit system for the detection of.
Cultures were tested on the day of mgit positivity with the sd tb ag mpt64 rapid test to confirm the presence of mtbc. Comparison of lowensteinjensen and bactec mgit 960 culture for mycobacterium tuberculosis in people living with hiv. Many laboratories validate dst of the secondline drugs by bactec mgit 960 system. Deteksi mikobakterium menggunakan manual mgit dan bactectm mgittm 960 system 1. Pdf to evaluate the performance of an automated bactec mgit 960, a nonradioactive. For the antimycobacterial susceptibility testing of mycobacterium tuberculosis i. Bd bactec mgit tubes use fluorometic technology for accurate results. However, the algorithm used for these assays in zambia has not yet been implemented and the most widely used drug susceptibility testing dst method remains mgit dst.
Susceptibility testing with the manual mycobacteria growth. A total of 9,558 specimens were investigated, comprising 3,074 body fluids, 1,878 tissues, and 2,069 urine samples, from which the recovery of mycobacteria was not yet established for mgit. Evaluation of the effectiveness of bactec mgit 960 for the detection of mycobacteria in bangladesh mehedi hasan a,b, saurab kishore munshi a, mst. Panta antimicrobial mixture for the mgit 960 tubes. Time to detection of the growth of mycobacterium tuberculosis in mgit 960 for determining the early bactericidal activity of antituberculosis agents. Oxygen dissolved in the media quenched the fluorescence in the bottom of the tubes. Oct 01, 2000 read comparison of bactec mgit 960 and bactec 460 for culture of mycobacteria, diagnostic microbiology and infectious disease on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Pdf comparison between the bactec mgit 960 system and. Pdf use of bactec mgit 960 for recovery of mycobacteria. Author links open overlay panel mehedi hasan a b saurab kishore munshi a mst.
Mycobacteria growth indicator tube see availability. Missed rifampicin resistance on the mgit 960 system has been. Rapid diagnosis of tuberculosis and multidrug resistance. Evaluation of the effectiveness of bactec mgit 960 for the detection of mycobacteria in bangladesh. The accuracy of the bactec mgit 960 system for susceptibility testing of 177 clinical isolates of mycobacterium tuberculosis to first line drugs isoniazid, rifampicin, ethambutol and streptomycin was compared with the agar reference method. Manual of clinical microbiology, 6th ed murray pr, baron ej, pfaller ma. Bd bactec mgit mycobacteria growth indicator tubes bd. Background combination of genotypic assays xpert mtb rif and mtbdrplus lipa would be a powerful tool to shorten the time for diagnosis of mdr tuberculosis tb. Research article evaluation of mgit 960 system for the. Comparative evaluation of the bactec mgit 960 system with. Lj solid culture and mycobacteria growth indicator tube mgit liquid culture systems and culture.
The results of susceptibility to rmp were all in agreement. The lower agreement found between the two methods suggests the unreliability of the bactec mgit 960 system for the drugs tested. Advanced fluorometric manual mycobacteria detection. For tb complex isolates, identification and drug susceptibility testing will be performed, as well as molecular detection of drug resistance. However, contamination rates were much higher for the mgit media, 1724%, compared to 7% for bactec, suggesting that contamination was a major cause of mgit reduced sensitivity.
Pdf evaluation of the bactec mgit 960 system for the. Apr 11, 20 at the national veterinary services laboratories the bactec mgit 960 and 4 solid media formulations were compared with the bactec 460 tb system on 6,795 veterinary diagnostic specimens submitted for mycobacterium bovis culture. Evaluation of mgit 960 system for the secondline drugs susceptibility testing of mycobacterium tuberculosis hyejinkim, 1 minjiseo, 2 youngkilpark, 1 jaeilyoo, 3 yeongseonlee, 3 gyungtaechung, 3 andsungweonryoo 1 korean institute of tuberculosis, osong sangmyung ro, osongeup, cheongwongun, chungbuk, republic of korea. Research article macrocolonies granules formation as a cause of falsenegative results in the mgit 960 system. This study evaluated a more rapid and costeffective drug susceptibility testing dst protocol using primary isolates of m. Pdf comparison between the bactec mgit 960 system and the. Evaluation of the bactec mgit 960 in comparison with.
The performances of two continuously monitoring mycobacterial culture systemsesp culture system ii esp ii. Modified protocol for drug susceptibility testing of mgit. Evaluation of the fully automated bactec mgit 960 system for. The bactec mgit 960 pza kit is used with the bactec mgit 960 system. In the present study, few of the strains were resistant to fluoroquinolones and further dst for this group is required. Pdf correlation between the bactec mgit 960 culture. Use of the bactec mycobacteria growth indicator tube 960. Its performance was compared to those of the radiometric bactec 460 instrument and eggbased. Bbl mgit mycobacteria growth indicator tube 7 ml with bd. Comparison of line probe assay to bactec mgit 960 system for. Phage lysin as a substitute for antibiotics to detect. The bactec mgit 960 system showed acceptable sensitivity for emb, str, and ofx. Pdf evaluation of mgit 960 system for the secondline. The range of concentrations used for each drug is the same as in the agar dilution method.
The objectives of this study were to i compare agreement of the mgit 960 system for firstline drugs with a methodology the resistance ratio method rrm that had been used in clinical trials, relating drug susceptibility to clinical outcome. Evaluation of automated bactec mgit 960 system for testing. At the national veterinary services laboratories the bactec mgit 960 and 4 solid media formulations were. Bactec mgit 960 system principle, manual and brochure the bactec mgit 960 mycobacterial detection system is the worlds first automated system for highvolume mycobacteria growth, detection and susceptibility testing from becton dickinson bd. Bbl mgit system is the manual system while bactec mgit 960 mgit 960 is the fully automatic system for detection of mycobacterial growth and drug susceptibility testing of m. Testing the critical concentration of six secondline drugs was performed by mgit 960 and compared with. Cause analysis and correlation with mycobacterial species. A rapid detection of resistance in mycobacterium tuberculosis is crucial for management and control of tuberculosis. It is specially designed to accommodate mycobacteria growth indicator tube mgit and incubate them at 37c. Performance of mycobacterium growth indicator tube bactec 960. Once grown, mycobacteria can be easily removed from the tube for further testing. Dr cetrangolo hospital, buenos aires province, argentina. Evaluation of bactec mgit 960 system for recovery of. Performance of the bactec mgit 960 compared with solid media for detection of mycobacterium in bangkok.
In fact, in 11 cases, afb was identified on the smears a few days after the signal in bactec mgit 960 was positive. The bactec mgit 960 instrument is a fully automated system that exploits the fluorescence of an oxygen sensor to detect growth of mycobacteria in culture. Deteksi mikobakterium menggunakan manual mgit dan bactectm. Use of mgit 960 for rapid quantitative measurement of the susceptibility of mycobacterium tuberculosis complex to ciprofloxacin and ethionamide. The objective of this study is to evaluate the critical concentration and perform dst for the 2nd line drugs. Evaluation of the bactec mgit 960 system in the detection of. Correlation between the bactec mgit 960 culture system with genotype mtbdrplus and tbsprint in multidrug resistant mycobacterium. Comparison of the bactec mgit 960 and esp culture system ii. Phage lysin as a substitute for antibiotics to detect mycobacterium tuberculosis from sputum samples with the bactec mgit 960 system b. Culture by the manual mgit system bbl mgit specimens were decontaminated and digested as described above for lj culture. The bd bactec mgit 960 system is for highvolume mycobacteria growth, detection and susceptibility testing and is designed to meet the needs of medium and highvolume laboratories. The tb retreatment patients with bactec mgit culture positive samples were protocolsprocedures, quality assurance planning and for 6 weeks in the bactec mgit 960 system becton in multivariate analyses, a manual backward.
The bactec mgit 960 system is an accurate and reliable method for rapid drug susceptibility testing dst of mycobacterium tuberculosis against secondline drugs. May 10, 2017 nonradiometric liquid culture methods called mycobacterium growth indicator tube mgit. The new bd bactec mgit 960 system is the next generation in a proven line of mycobacteria testing instruments from bd biosciences the undisputed world leader in mycobacteriology. The bd bactec mgit 960 system builds on the legacy of the bd bactec 460tb system, the worlds first instrumented system for mycobacteria testing. Technology advancement l the bactec mgit 960 system detects organism growth by measuring fluorescence from the bottom of a mgit paratb medium tube. Comparison of the bactec 460tb system and the bactec mgit 960. This site is like a library, you could find million book here by using search box in the header. This mgit 960 system is a fully automated, nonradiometric, and used to incubate and monitor 960 samples at a time with automated resultreporting system. The bactec mgit 960 results were compared with those obtained with the radiometric bactec 460tb system, and when there was disagreement, the method of proportion on agar plates was used as a reference.
Evaluation of the bactec mgit 960 tb system for recovery and identification of mycobacterium tuberculosis complex in a high volume tertiary care centre c rodrigues, s shenai, m sadani, n sukhadia, m jani, k ajbani, a sodha, a mehta. The critical concentration established for levofloxacin and amikacin was 1. Pdf use of mgit 960 for rapid quantitative measurement. Comparison of the mgit 960, bactec 460 tb and solid media for. Mycobacteriology laboratory manual v list of abbreviations and acronyms afb acid fast bacilli ast antimycobacterial susceptibility testing. All books are in clear copy here, and all files are secure so dont worry about it.
Evaluation of mgit 960 and the colorimetricbased method f. Comparison of the bactec 460tb system and the bactec mgit 960 system in recovery of mycobacteria from clinical specimens author. The mgit mycobacteria growth indicator tubes contain 4 ml of middlebrook 7h9 broth base with a fluorescent indicator and is intended for the manual detection and recovery of mycobacteria. Bd bactec mgit tubes fit into standard procedures for decontamination naclnaoh and waste disposal. The aim of this study was to investigate the prevalence of falsenegative culture sample in beijing and the potential factors associated with the detection failures by mgit 960. Drug susceptibility testing of mycobacterium tuberculosis. The reliability of the mycobacteria growth indicator tube mgit 960 system for. Research article macrocolonies granules formation as a. The following tables define the astm 94 request record fields that are transmitted from the lis in an download message and the mgit 960 statuses. The bd bbl mgit mycobacteria growth indicator tube supplemented with bd bactec mgit growth supplement and bd bbl mgit panta antibiotic mixture is intended for the detection and recovery of mycobacteria using the bd bactec mgit 960 and bd bactec mgit 320 systems. Bd bactec mgit tubes only require the addition of the bd bactec mgit 960 supplement prior to specimen inoculation and incubation.
The mgit 960 tubes were incubated at 37 6 2uc in the bactec mgit 960 instrument. Factors associated with missed detection of mycobacterium. From 1985 to 1992, the number of reported cases of tuberculosis increased 18%. Elisa schena, lubov nedialkova, emanuele borroni, simone battaglia, andrea maurizio cabibbe, stefan niemann, christian utpatel, matthias merker, alberto trovato, sabine hofmannthiel, harald hoffmann, daniela maria cirillo, delamanid susceptibility testing of mycobacterium tuberculosis using the resazurin microtitre assay and the bactec mgit 960 system, journal of antimicrobial. We have developed a method combining the bactec mgit 960 rapid culture system with the is6110based pcr for rapid diagnosis of tuberculosis. Comparison of the recovery of mycobacterium bovis isolates. We evaluated the performance of bactec mgit 960 for automated testing of the susceptibility of 3 strains of mycobacterium tuberculosis to streptomycin, isoniazid, rifampin, and ethambutol. Bd bactec mgit 960 instrument specifications scenario 1 scenario 2 scenario 3 scenario 4 instrument plus printer instrument plus printer plus pc instrument plus printer plus pc plus ups instrument plus printer plus pc plus line conditioner dedicated 15 amp line 20 amp line if pc is colocated on same line as instrument or printer 20 amp line. Bactec mgit 960 system principle, manual and brochure.
We standardised rnitro benzoic acid pnba assay on mgit 960 tb system for identification of m. The bd bactec mgit 960 system is the next generation in a proven line of mycobacteria testing instruments from bd diagnostic systems the undisputed world leader in mycobacteriology. Direct drug susceptibility testing of mycobacterium. All 716 37c in mgit 960 system till positive or for 42 days. Sabiha banu momi b, farjana rahman a, rashed noor a, a department of microbiology, stamford university bangladesh, 51 siddeswari road, dhaka 1217, bangladesh b mycobacteriology laboratory, international centre for diarrheal disease research. Direct drug susceptibility testing of mycobacterium tuberculosis for rapid detection of multidrug resistance using the bactec mgit 960 system. Comparison of lowensteinjensen and bactec mgit 960 culture. Of a loopfull inoculated on the lowensteinjensen lj the positive tubes, 560 78. A query contains a set of request parameters that are used to determine which specimens to access. Evaluation of the bactec mgit 960 system for the recovery of mycobacteria. Introduction pyrazinamide pza is an important firstline drug used in combination with other tuberculosis tb drugs and has a critical role in shortening. Use of bactec mgit 960 for recovery of mycobacteria from.
Performance of the bactec mgit 960 compared with solid. Failure of bactec mgit 960 to detect mycobacterium tuberculosis complex within a 42day incubation period. Comparison of the bactec mgit 960 and bactec 460tb. Becton dickinson system have been introduced for better recovery of mycobacterium.
The bd bactec mgit system maximizes productivity without depleting precious resources, including additional equipment, storage and incubator capacity. Read online mgit report on the important events organized. Pdf evaluation of the bactec mgit 960 tb system for recovery and. Evaluation of bactec mgit 960 system for recovery of mycobacterium tuberculosis complex in pakistan luqman satti, aamer ikram, shahid abbasi, tariq butt, nasarullah malik and irfan ali mirza. Pdf failure of bactec mgit 960 to detect mycobacterium. Despite these issues, the mgit 960 system is still favored over the use of solid media due to. Procedure manual for bactec mgit 960 tb system read download xdrtb in clinical isolates from four highburden countries. We would like to show you a description here but the site wont allow us. Evaluation of mgit 960based antimicrobial testing and. Another problem when dealing with bactec mgit 960 is the frequent disagreement, albeit temporary, between the positivity reported by the system and the lack of afb in the smear.
The broth is contained in a plastic tube with screwtop cap and supplemented with bd mgit oadc enrichment and bd mgit panta antibiotic mixture. Research article open access comparison of the mgit. Evaluation of the bactec mycobacteria growth indicator tube mgit 960 system and the colorimetricbased method cmm for first and secondline drug susceptibility testing fldst, sldst against mycobacterium tuberculosis. Despite the demonstration of excellent performance, mycobacterial growth in bactec mgit 960 can go undetected. Comparative evaluation of the bactec mgit 960 system with solid medium for isolation of mycobacteria. Mdl test order mycobacteria culturing mgit only mgitbymail. Intended use the bactec mgit 960 pza kit is used as a rapid qualitative procedure for susceptibility testing of mycobacterium tuberculosis, from culture, to pyrazinamide pza. Detecting mycobacterium tuberculosis in bactec mgit 960 cultures. Use of mgit 960 for rapid quantitative measurement of the. Comparison of the bactec 460tb system and the bactec. Failure of bactec mgit 960 to detect mycobacterium.
Deteksi mikobakterium menggunakan manual mgit dan bactectm mgittm 960 system didik agus santoso,dr dr. Are the discrepancies always due to the test method. The bd bactec mgit 320 system is designed to help smallercapacity laboratories quickly and accurately detect tb. Comparison between the bactec mgit 960 system and the agar proportion method for susceptibility testing of multidrug resistant tuberculosis strains in a high burden setting of south africa. Incubation and automated growth detection in mgit 960. This instrument is produced by becton dickinson bd. Author links open overlay panel claudio scarparo a paola piccoli a alessandra rigon a giuliana ruggiero a paolo ricordi a claudio piersimoni b. Evaluation of mgit 960 system for the secondline drugs. Multicenter evaluation of bactec mgit 960 system for. Numerous studies have been carried out using mgit system for primary isolation of mycobacteria as compared with lj and ogawa. The bactec mycobacteria growth indicator tube 960 mgit 960 system was applied for recovery of mycobacteria from extrapulmonary specimens and compared with solid media lowensteinjensen and stonebrink. Pdf time to detection of the growth of mycobacterium.
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